Answer:
The single guide RNA forms hydrogen bonds with DNA, while Cas9 hydrolyzes phosphodiester bonds
Explanation:
The base pairing between nucleic acid strands (either DNA or RNA) is through hydrogen bonds between nucleotide bases. In DNA, Adenine always forms two hydrogen bonds with Thymine, while Guanine always forms three hydrogen bonds only with Cytosine. Moreover, adjacent nucleotides in the same strand are covalently linked by phosphodiester bonds (i.e., covalent bonds between the 5' phosphate group of one nucleotide and the 3'-OH group of another). The CRISPR/Cas9 genome editing systems make use of single-guide RNAs (sgRNAs) that interact with DNA through hydrogen bonds. These sgRNAs have perfect complementarity to the target DNAs in order to bind them. On the other hand, Cas9 is an enzyme that hydrolyzes phosphodiester bonds in both DNA strands very precisely and accurately by using a sgRNA complementary to a specific DNA sequence.
The correct order of flow of energy and its conversion is
- Potential and Kinetic energy
During photosynthesis, plants converts light energy from the Sun into
chemical energy in the form of glucose. Cellular respiration involves the
conversion of chemical energy from glucose into potential and kinetic
energy by body cells
The chemical energy from glucose is converted into ATP which is another
form of chemical energy.The ATP formed helps cells perform various
activities and state of rest such as sleeping in the body through the
conversion of the chemical energy into kinetic and potential energy
respectively.
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Answer:
Explanation:
Pausing can be detected by gel electrophoresis and autoradiography in experiments which involves the transcription into Vivo with radiolabeled nucleotides. After incubation isolate the labelled RNA products and subject to gel electrophoresis and audio radiography. Any paused species will definitely accumulate and can be detected.
