Answer:
it is expected that the mutation results in a reduced initiation of translation and thereby decreasing the level of the protein A, while it does not change the level of mRNA A
Explanation:
Translation in bacteria starts with the formation of the initiation complex which is composed of the small ribosomal subunit, the messenger RNA (mRNA), initiation factors and the initiator transference RNA (tRNA) containing N-formyl-methionine. The small ribosomal subunit binds to a polypurine stretch of variable length in the mRNA called 'the Shine-Dalgarno sequence'. A mutation in this sequence reduces the affinity of the ribosome for the mRNA, thereby, in this case, decreasing the level of protein A. Since transcription occurs before translation, it is expected that this mutation does not change the level of expression of the mRNA A.
The answer would be D, 7 hours of sleep
Due to arising conflict in self-image and others.
In Psychology, self-actualization is process where an individual reaches and achieves their full potential and capabilities as a person. In Maslow's model, this is considered to be the highest step in the hierarchy.
Now, most individuals do not achieve this because of conflict that occurs within the self and with other people.
Iced tea's energy increases while the energy of the glass decreases.
<h3>What is energy?</h3>
Energy is the capacity for doing work. It may exist in potential, kinetic, thermal, electrical, chemical, nuclear, or other forms
Iced tea's energy increases while the energy of the glass decreases because the ice is getting warmer so the glass is also getting warmer. meaning that the glass is decreasing in energy while the ice tea is increasing in energy.
Hence, iced tea's energy increases while the energy of the glass decreases.
Learn more about the energy here:
brainly.com/question/1932868
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Answer:
The correct answer is b.Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
Explanation:
If I have a very small amount of gene for a fluorescent protein than the first step is to amplify the gene so that appropriate protein can be produced. PCR is the instrument that is used to amplify the protein.
So after amplification of the gene, the plasmid vector will be used in which the gene will be inserted because the plasmid vector is used to insert this gene in host cells where protein will be expressed.
The final step will be to transform bacteria with recombinant plasmid so that plasmid can make its copy and express a fluorescent protein in bulk.
