<h2>CRISPR/Cas9</h2>
Explanation:
CRISPR can be used to reintroduce dystrophin back into the KO mouse
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats and is used to for gene editing
- CRISPR/Cas-mediated genome editing has been shown to permanently correct DMD mutations and restore dystrophin function in mouse models
- Germline editing by injecting zygotes with CRISPR/Cas9 editing component was first done in mdx mice by correcting the mutated exon 23
- Postnatal editing of mdx mice was then achieved using recombinant adeno-associated virus to deliver CRISPR/Cas9 genome editing components and correct the dystrophin gene by skipping or deleting the mutated exon 23 in vivo
- Germline and postnatal CRISPR/Cas9 editing approaches both successfully restored dystrophin function in the mice and same technique can be used for KO mouse model
Answer:
D) a scientific journal about chemistry and a biography of a famous chemist
Answer: The DNA on the paternal copy of the chromosome will be methylated at the imprinting center, while the DNA on the maternal copy of the chromosome will not be methylated in this region.
Explanation:
Chromosomes are DNA structures associated with proteins such as histones. They are found in the nucleus of cells and contain genetic information for the development and functioning of an organism. Human beings are diploid, meaning that they possess two copies of each of the 23 chromosomes (a total of 46).
Gametes, which are sex cells such as the sperm (produced by the male) and the egg (produced by the female) that are haploid. This means that they possess only one chromosome of each pair. During fertilization, a male gamete fuses with a female gamete to generate a zygote, which will give rise to a new human being. <u>This new individual will possess half of the genetic material from its father and half from its mother. Thus having a total of 46 chromosomes, 23 from each parent</u>.
Genetic imprinting modulates gene expression by chemical modification of DNA and/or modification of chromatin structure. Often, genetic imprinting causes a gene to be expressed only on the chromosome inherited from one of the parents. One example of imprinting is DNA methylation, which is a process by which methyl groups are added to DNA. <u>Methylation modifies DNA function when found in the promoter gene, repressing gene transcription. This means that a methylated gene will not be expressed</u>, that is, it will not produce a protein encoded by that gene. So, if a region of DNA is imprinted in the sperm cell, the paternal chromosome inherited from this sperm will be methylated in the genes of the offspring. And the offspring will only express the maternal copy inherited, which will not be methylated.
They both have a nucleus. They both have cytoplasm.
