I believe the pellet is discarded in this part of protein purification procedure because the pellet contains unwanted bacterial debris, these includes the bacterial cell walls, membranes, and chromosomal DNA. Additionally, the pellet contains little, if any, GFP and can be discarded. Protein purification is a procedure that is intended to isolate one or few proteins from a complex mixture, normally cells, tissues or whole organisms. The process is important for the purpose of characterization of the function, structure and interactions of the proteins of interest.
As scientist- you are taught to question Everything. you make a hypothesis and gather data that either proves the hypothesis to be true or refutes your hypothesis.
a theory is a deliberately tested and studied hypothesis pointing to a collection of data that proves a hypothesis to be true. (or remain unrefuted)
a theory isn't some random idea not backed by data.
Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body. Chromosomes are structures within cells that contain a person's genes. Genes are contained in chromosomes, which are in the cell nucleus.
Hope it’s right
Best luck with your studying