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Kobotan [32]
3 years ago
13

DNA Polymerase helps copy a DNA molecule during the process of what?

Biology
1 answer:
PtichkaEL [24]3 years ago
7 0
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from one original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.

Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.

Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double stranded DNA to give two single strands of DNA that can be used as templates for replication.

DNA polymerase adds new free nucleotides to the 3’ end of the newly-forming strand, elongating it in a 5’ to 3’ direction. However, DNA polymerase cannot begin the formation of this new chain on its own and can only add nucleotides to a pre-existing 3'-OH group. A primer is therefore needed, at which nucleotides can be added. Primers are usually composed of RNA and DNA bases and the first two bases are always RNA. These primers are made by another enzyme called primase.

Although the function of DNA polymerase is highly accurate, a mistake is made for about one in every billion base pairs copied. The DNA is therefore “proofread” by DNA polymerase after it has been copied so that misplaced base pairs can be corrected. This preserves the integrity of the original DNA strand that is passed onto the daughter cells.



A surface representation of human DNA polymerase β (Pol β), a central enzyme in the base excision repair (BER) pathway. Image Credit: niehs.nih.gov

Structure of DNA polymerase

The structure of DNA polymerase is highly conserved, meaning their catalytic subunits vary very little from one species to another, irrespective of how their domains are structured. This highly conserved structure usually indicates that the cellular functions they perform are crucial and irreplaceable and therefore require rigid maintenance to ensure their evolutionary advantage.

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Explanation:

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2 years ago
1) How is DNA condensed to form a chromosome?
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Explanation:

1) DNA forms a DNA/protein complex called chromatin. It does this by wrapping around histone proteins. These histone proteins are usually present in the form of a nucleosome, which is a unit containing 2 copies of 4 histones (H2A, H2B, H3 and H4). The chromatin fibre at its most compact forms tightly coiled structures called chromosomes. These structures are only present during cell division. When the cell is in interphase (i.e. not dividing), the chromatin is not as tightly condensed, and instead the chromatin is more relaxed to allow the genes within to be expressed.

2) Genes are the functional units that the cell uses to make RNA and protein. The genes are first transcribed into RNA, which is processed and then translated into a polypeptide chain, which forms a complete protein that performs activities in the cell/tissue/organism. However, the whole genome does not form genes, there are regions that do not correspond to a gene. These regions are called "non-coding DNA" or sometimes even "junk DNA". However, that does not mean that these regions do not have important roles. The role of this DNA is usually in regulating the activity of the nearby genes. This DNA might contain important regulatory sequences such as promoters/enhancers/silencers that control how the gene is used by the cell, by for example, recruiting transcription factors or silencing proteins.

3) The genetic code is the language used by the cell. It explains how the cell can transcribe the information in the DNA, to RNA, process the RNA, and then translate the RNA into a polypeptide, and eventually a mature protein. In contrast, gene expression represents how the cell actually uses this information. Not all the genes are transcribed at the same time, instead, the activity of genes is carefully controlled to produce appropriate gene expression patterns, allowing the cell to properly perform its functions. Gene expression is hugely different between cells in an organism, for example the gene expression patterns of a muscle cell will  be hugely different to that of a blood cell.

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7 0
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