Question

Explain why you can't fully test the lipase activity in tube 5.

Answer 1

It has a very low pH already , so the test requires the visualization of a diminishing in pH, it may be hard to identify.   
The ideal pH of gastric lipase is 3 – 6, implying that gastric lipase does not work at its ideal pH in the stomach   
A lipase is any catalyst that catalyzes the hydrolysis of fats (lipids). Lipases are a subclass of the esterases. Lipases perform basic parts in the absorption, transport and preparing of dietary lipids

Answer 2

The  reason  why  you  cant  test  the  activity  of  Lipase in  test  tube  5  is  because in test  tube  5  the  PH  is  low.  This  decrease  in  pH  might  make   it  difficult  to  detect  the  activity  of  lipase  since  the  optimal  ph  of   pancreatic  lipase  is  around  8.0.  generally  high   or   low  PH  result   to  complete  loss  of  activities  of  most  enzymes.