Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
In one of the studies of about rats in Easter Island, it was believed that rats were the reason for the decrease of palm trees.
According to a system framework study, there are relationships between the birth and death of people with the rats and the palm trees. It explains that peoples' population in Easter island has a relationship with rats and palm trees in the area.
<span>The number of palm trees decreases because of the increased number of rats that eats its fruits. Seed germination became frequently interfered because of this activity.
</span><span>However, the slow growth of palm trees was also blamed for the tree cutting off the people.</span>
Natural selection is the right answer hope this helps.
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Answer: C). prolong the effect of epinephrine by maintaining elevated cAMP levels in the cytoplasm
Explanation: In the epinephrine pathway, binding of epinephrine to its receptor triggers a conformational change in the receptor and the interaction of the receptor with its associated Gs protein. This interaction causes the replacement of GDP bound to Gs protein with GTP thus activating the Gs protein. The activation of the Gs protein causes the alpha subunit of the Gs protein to dissociate and move to adenylyl cyclase, another membrane protein in the pathway. The association of the alpha subunit of the Gs protein with adenylyl cyclase activates adenylyl cyclase which in turn catalyzes the synthesis of cyclic AMP (cAMP) a second messenger. cAMP is quickly degraded to 5'-AMP by an enzyme phosphodiesterase. Inhibition of the activity of phosphodiesterase will increase the half life and the cytoplasmic level of cAMP thus potentiating the action of epinephrine.
