The "scissors" of the molecular biology are: DNA Restriction enzymes.
A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.
Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.
The "glue" of the molecular biology are: DNA ligase
In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.
The use of these tools in molecular biology: Cloning
Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).
Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.
A memory B cell is kinda of how it sounds it has a memory of what your body has fought before such as a virus and can stimulate a response quicker then the first time you encountered it.
If you lack memory B cells your body doesn't built up the same immune response when you come into contact with the virus again.
"Vaccination would prevent further disease" Incorrect, a vaccine lets you build up memory cells to combat the disease if you lack them you cannot build up a defense.
"Vaccination would not prevent further disease" Correct, a vaccine can be a dead version of a virus or parts of a virus that shows your body what to respond to quickly in the future. If you body cannot make memory b cells it wouldn't help to use a vaccine. Your body wouldn't keep a memory of it being bad.
"You would not produce antibodies" Even if your body does not remember a particular virus it does produce antibodies to combat it just not as quick or efficiantly as something it has come into contact with before and produced memory cells.
"You would over produce histamine" Incorrect, different process for that histamine.
The correct answer for this question would be A) Nnew = Nold.
<span>After ovulation, an oocyte, or egg cell, remains viable in the female body for up to 24 hours. The generally accepted lifespan in a woman's body is 12 to 24 hours for the egg and up to five days for the sperm cells. Intercourse occurring as many as five days prior to ovulation, therefore, could potentially result in pregnancy.</span>
