Cation-exchange chromatography is used when the molecule of interest is positively charged on the neutral pH. The stationary phase is than negatively charged and positively charged molecules are loaded to be attracted to it. So, positively charged peptides will elute the last.
Intercostal Muscles
The muscles of respiration
are muscles that contribute to exhalation and inhalation by causing the contraction
and expansion of the thoracic cavity. However, diaphragm is the main muscle of breathing
while intercostal muscles is one of the most important muscles for respiration.
Intercostal muscles function by manipulating the width of the rib cage and they
are attached between the ribs.
Answer:
It could lead to several later problems in the cell.
Explanation:
Removing a piece of DNA could severely affect the cell but it could also cause nothing. Depending on the part that is damaged, it could codify for a vital protein but it could also be an intron, which are pieces of DNA that don't codify for any protein. This has been recently researched and even though there are introns that could cause some serious trouble, most of them are an unknown matter to nowadays scientists. Anyway, a wrongly repaired sequence could end in a mutation that can potentially damage the cell and therefore the organism.
Although the DNA packaging with histones proteins is mean to form nucleosomes (groups of 8 histones proteins), the higher structures why the genetic material is packaged with those molecules is to form chromosomes, as you said.
