Answer:
the diagram explains the process of DNA digestion and DNA ligation, which is usually used in molecular cloning techniques
Explanation:
Molecular cloning can be defined as the process used to synthesize multiple copies of a particular DNA fragment. Molecular cloning requires the insertion of a foreign DNA fragment into an appropriate vector (e.g., a plasmid) through the action of specific enzymes that serve to cut and ligate DNA fragments. DNA digestion and DNA ligation use specific restriction enzymes and DNA ligases, respectively, in order to insert the foreign DNA fragment. For this purpose, restriction enzymes that generate single-stranded overhangs are preferred to create sticky ends which bind by complementary base pairing. Subsequently, a DNA ligase enzyme joins the DNA fragments together in order to create recombinant DNA molecules. DNA Ligation is often achieved by using a specific T4 DNA ligase, while there are many restriction enzymes that generate sticky-ends (e.g., BamHI, EcoRI, BaI228I, etc).
It is known as a transgenic organism. This procedure is otherwise called "genetic engineering." Genes of one species can be altered, or qualities can be transplanted starting with one animal types then onto the next. Genetic engineering is made conceivable by recombinant DNA innovation. Living beings that have modified genomes are known as transgenic.
The correct answer is monohybrid cross
Answer:
1. B. parasitism
2. C. parasitism, predation, mutualism
Explanation:
Answer:
The smallest size tube that will hold the entire reaction is 0.5ml
Explanation:
25ul (DNA sample) + 25ul (enzyme) + 50ul (buffer) + 400ul (water) = 500ul total
since 1000ul=1ml, then 500ul = 0.5ml
Answer:
The device that mostlikely would not be used P20
Explanation:
P20 is a pipette used to measure and sample volumes of 20ul maximum.
Answer:
The reaction mix will have 400 units of the enzyme.
The working dilution will be 0.8 Units/ul.
Explanation:
The mix has 20ul of the enzyme which is 20Units/ul. Then we have 20ulx20Units/ul= 400 Units of enzyme in the mix.
Since the final volume is 500ul, the final concentration 400Units/500ul=0.8 Units/ul.
