Because of increasing in the temperature causing decrease of amount of oxygen that dissolved in water
I believe the answer is D , but I’m not 100% sure
Answer:
1.5 mM
Explanation:
<u>PCR mix</u>
- 5 µL Primers
- 1.0 µL dNTPs
- 1.0 µl Taq pol
- 10.0 µl of 5X buffer
- 20.0 µl DNA
- 3.0 µl MgCl₂ 25mM
Total volume = 50 µL
<u>We can use the dilution factor (df) to calculate the final concentration of MgCl₂</u>
<h2>
![\frac{Initial\ concentration}{dilution\ factor} = final\ concentration](https://tex.z-dn.net/?f=%5Cfrac%7BInitial%5C%20concentration%7D%7Bdilution%5C%20factor%7D%20%20%3D%20final%5C%20concentration)
</h2>
The dilution factor can be calculated as the final volume divided by the sample volume.
- Final volume = 50 µL of PCR mix
- Sample volume = 3 µL of MgCl₂
- Dilution factor = 50 µL / 3 µL = 16,67
<u>The final concentration of MgCl₂ in the mix will be:</u>
![\frac{25\ mM}{16.67} = 1.5\ mM](https://tex.z-dn.net/?f=%5Cfrac%7B25%5C%20mM%7D%7B16.67%7D%20%3D%201.5%5C%20mM)
False, Plants makes lipids also
It going to be day and night