A scientific law should have no exceptions otherwise the law has failed
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
My teacher always taught me the saying "(A)pples on (T)rees, (C)ars in (G)arrages, meaning that A always went with T and C always goes with G
When that applies here, 30 Red, or Adenine legos would go with 30 Thymine legos, which is the color orange
30 reds would go with 30 oranges in this case
