Yes...one dog has been observed to kill themselves purposefully.
Answer:
In this case, the question asked is "To what lengths must we go to protect plants and animals?", which is not specific and cannot be used for scientific research.
The ecological researches for the safety of the environment involves the protection of the plants and the animals on the basis of their number and vulnerability to extinction. Almost all the length of the plants are ecologically important and can decrease in number due to the changes in the environment, the length is not a parameter to determine the protection boundary.
Answer: The bacteria transformed with this particular plasmid will form white colonies on the plates containing ampicillin and Xgal.
Explanation: The lacZ gene produces an enzyme called β-galactosidase which is responsible for the breakdown of lactose into glucose and galactose. The lacZ gene is one of the three genes (the other two being lacA and lacY) of the lac operon which is responsible for the transport and mechanism of lactose in E. coli and many other bacteria.
In recombinant DNA technology, when a plasmid is to be used to transform a host cell, such markers are used to help screen the transformed cells from the ones that have not taken up the plasmid. Xgal present in the plates is an artificial substrate which is hydrolyzed by
β-galactosidase into 5-bromo-4-chloro-indoxyl which will dimerize and oxidise into 5,5'-dibromo-4,4'dichloro-indigo. This is a blue pigment which will give blue color to the bacterial cells. Introducing a DNA fragment in this lacZ gene will make it non-functional so it will not be able to produce the enzyme.
Therefore, when a bacterial cell is transformed with a plasmid containing ampicillin resistance gene and a DNA fragment introduced in the lacZ gene and then grown on plates containing ampicillin and Xgal, white colored colonies will appear. The white colonies will show the bacterial cells that have successfully taken up the plasmid with the DNA fragment incorporated in the lacZ gene as this will render the gene non-functional and will not produce β-galactosidase which will breakdown Xgal to give blue colonies. Since the plates contain ampicillin, only the bacterial cells that have been successfully transformed with the plasmid ( the ones that have the DNA fragment and the ones without it) will grow as the ampicillin resistance will give them resistance against ampicillin in the plates. The bacterial cells that have not taken up the plasmid will not be resistant to ampicillin and will not form colonies on the plate.
This is called blue-white screening which is used to identify successfully transformed host cells. A picture of this is given in the attachment, taken from the following website:
https://www.mun.ca/biology/scarr/Blue_&_White_Colonies.html
Growth and repair
creation of gametes (sex cells)
method of reproduction in unicellular organisms
Binary Fission - type of reproduction that occurs in bacterial cells, single celled organism splits and becomes two identical organisms
Chromosomes and DNA
Chromosomes are DNA wrapped around proteins to form an X-shaped structure.
The diagram will help you see the relationship.
1. Chromosomes are found in the nucleus
2. Chromosomes are made of DNA
3. Sections of chromosomes are called genes
DNA - deoxyribonucleic acid (it is the genetic code that contains all the information needed to build and maintain an organism)
Chromosome Structure
Chromosome Numbers
Each organism has a distinct number of chromosomes, in humans, every cell contains 46 chromosomes. Other organisms have different numbers, for instance, a dog has 78 chromosomes per cell.
Somatic Cells - body cells, such as muscle, skin, blood ...etc. These cells contain a complete set of chromosomes (46 in humans) and are called DIPLOID.
Sex Cells - also known as gametes. These cells contain half the number of chromosomes as body cells and are called HAPLOID
Chromosomes come in pairs, called Homologous Pairs (or homologs). Imagine homologs as a matching set, but they are not exacly alike, like a pair of shoes.
Diploid cells have 23 homologous pairs = total of 46
Haploid cells have 23 chromosomes (that are not paired) = total of 23
Homologous Chromosomes
Sex Determination
Chromosomes determine the sex of an offspring. In humans, a pair of chromosomes called SEX CHROMOSOMES determine the sex.
If you have XX sex chromosomes - you are female
If you have XY sex chromosomes - you are male
During fertilization, sperm cells will either contain an X or a Y chromosome (in addition to 22 other chromosomes - total of 23). If a sperm containing an X chromosome fertilizes an egg, the offspring will be female. If a sperm cell containing a Y chromosome fertilizes an egg, the offspring will be male.
Creation of a Zygote
When two sex cells, or gametes come together, the resulting fertilized egg is called a ZYGOTE
Zygotes are diploid and have the total 46 chromosomes (in humans)
Karyotype
A karyotype is a picture of a person's (or fetus) chromosomes. A karyotype is often done to determine if the offspring has the correct number of chromosomes. An incorrect number of chromosomes indicates that the child will have a condition, like Down Syndrome
Compare the Karyotypes below
Notice that a person with Down Syndrome has an extra chromosome #21. Instead of a pair, this person has 3 chromosomes - a condition called TRISOMY (tri = three)
Trisomy results when chromosomes fail to separate - NONDISJUNCTION - when sex cells are created. The resulting egg or sperm has 24 instead of the normal 23.
Other conditions result from having the wrong number of chromosomes:
Klinefelters Syndrome - XXY (sex chromosomes)
Edward Syndrome - Trisomy of chromosome #13
