Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
When temperature is increased, molecules come together more frequently and with greater energy. Because chemical reactions need a certain amount of energy to occur, increasing the energy of the molecules involved in the reaction can speed up the rate at which the reaction occurs.
Answer: 4
Explanation:
i dont know but it looks right
Answer: The angle between the geographic and magnetic poles extends more towards the east, as you move to the north. The magnetic pole is actually near greenland.
