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Sladkaya [172]
3 years ago
13

The ligation you carried out in Laboratory 3 can result in a number of plasmids, but none of these have been replicated in a bac

terial cell. What configurations -- supercoiled , nicked circle, and multimer - might the ligated plasmids have ?
Biology
1 answer:
Ipatiy [6.2K]3 years ago
7 0

Nicked circle is the reason.

Explanation:

During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.

Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.

The ligation in the plasmid did not occur properly hence the replication is interrupted  hence no new plasmids with gene of interest replicated in the ligation carried out.

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They have short wings enabling them to fly with a lot of speed and
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3 0
4 years ago
the element nitrogen has an atomic number of 7 and an atomic mass of 14 how many protons, neutrons and electrons are in a atom o
Nat2105 [25]

Answer: By definition, if  the atomic number  = 7 , then there are 7 protons, 7 electrons, and 8 neutrons!

Explanation:

The atomic number is by definition the number of protons, positively charged particles, contained within the atomic nucleus. So if there are 7 protons, there MUST be 7 electrons, 7 negatively charged particles. Why? Because matter is electrically neutral, and positive and negative particles must be equal.

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3 0
3 years ago
What is the density of a liquid with a mass of 17.4 g and a volume of 20 mL?
iren [92.7K]

Answer:

The density of the liquid is 0.87 g/ml

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8 0
3 years ago
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2. About how much of the energy contained originally in the grass is usable by the
cestrela7 [59]

Answer:

The correct option is <em>B. 0.1 </em>

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The grass will give

1/100× 10

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6 0
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3 years ago
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