1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Sladkaya [172]
3 years ago
13

The ligation you carried out in Laboratory 3 can result in a number of plasmids, but none of these have been replicated in a bac

terial cell. What configurations -- supercoiled , nicked circle, and multimer - might the ligated plasmids have ?
Biology
1 answer:
Ipatiy [6.2K]3 years ago
7 0

Nicked circle is the reason.

Explanation:

During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.

Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.

The ligation in the plasmid did not occur properly hence the replication is interrupted  hence no new plasmids with gene of interest replicated in the ligation carried out.

You might be interested in
Help with the question in the image!
erma4kov [3.2K]
Most closely related to the Otariidae
8 0
3 years ago
research the following terms: gross, profit, total revenue, and gross profit margin .Make a diagram explaining these terms. Then
exis [7]

Answer:

uuiuuuuuijhj until j um internationalization

5 0
3 years ago
At sea level, the atmospheric pressure is ______ pounds per square inch.
AlexFokin [52]
The answer is c.....
4 0
2 years ago
Read 2 more answers
Is glucose reduced in the Krebs cycle?
kiruha [24]

Answer:

Thus, during the Citric Acid cycle, the breakdown of glucose into carbon dioxide is completed. There are four redox reactions, three of which yield reduced NADH and one FADH2. Thus, the oxidation of glucose is completed in the Kreb's cycle

8 0
3 years ago
Read 2 more answers
A scientist hypothesizes that if the inner membrane of a mitochondrion were smooth instead of folded, less ATP would be produced
I am Lyosha [343]

Answer:

i think its bcz

ATP if formed by deeply folded membrane

So if the membrane is smooth instead of folded

it will affect the ATP production

<h2>Im not that sure with the answer</h2>
6 0
2 years ago
Other questions:
  • What prevents the elodea cells from completely collapsing?
    14·1 answer
  • Research shows that Mount Everest grows and average of 4 millimeters each year. How many years will it take to grow 720 millimet
    10·2 answers
  • Which gastrointestinal hormone stimulates secretion of the components of hydrochloric acid?
    13·1 answer
  • have you ever cleaned and polish furniture or a car so that you could see your reflection? How did cleaning and polishing do tha
    15·1 answer
  • Explain role of allergens in allergies
    11·1 answer
  • A cold-tolerant and salt-tolerant type of crab would likely be found in which part of the ocean? question 13 options:
    11·1 answer
  • How do changes in nutrient affect biodivesity
    11·1 answer
  • Which of the following statements is generally true of aneuploidies in newborns? a. An aneuploidy resulting in the deletion of a
    12·1 answer
  • Explain the relationship between mutations, adaptations, natural selection, and extinction
    11·2 answers
  • How do you think meteorologists predicted the
    7·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!