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Sladkaya [172]
3 years ago
13

The ligation you carried out in Laboratory 3 can result in a number of plasmids, but none of these have been replicated in a bac

terial cell. What configurations -- supercoiled , nicked circle, and multimer - might the ligated plasmids have ?
Biology
1 answer:
Ipatiy [6.2K]3 years ago
7 0

Nicked circle is the reason.

Explanation:

During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.

Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.

The ligation in the plasmid did not occur properly hence the replication is interrupted  hence no new plasmids with gene of interest replicated in the ligation carried out.

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What type of dominance refers to a situation in which two alleles for the same gene are
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Answer:

complete

Explanation:

it's the best choice

8 0
2 years ago
Of all the species you observed in this lab, which is the most difficult to classify as fungus-like, plant-like, or animal-like?
GalinKa [24]

Scientists used to lump protists into a single kingdom, and they still use this classification for some purposes. However, science has largely recognized that the taxonomic grouping known as Kingdom Protista actually includes a wide range of organisms that are not particularly related. Biologists are currently in the process of revising their classification to reflect the evolutionary relationships among this massive set of organisms.

TL;DR (Too Long; Didn't Read)

Biologists used to classify all protists as part of Kingdom Protista, but almost every rule used to describe them has some major exception. They are now attempting to revise the classification of the organisms in this kingdom. Some scientists have tried to sort protists in comparison to three of the other kingdoms: plant, animal and fungus. This has not worked because many protists exhibit characteristics from two or more of these kingdoms. The current attempts of classification focus on grouping protists into between three and ten kingdoms, based on species’ common evolutionary ancestors.

Mind-Boggling Diversity

The protists exhibit considerable diversity. Almost every rule used to describe them has some major exception. Some protists live as parasites, others as predators and still others as producers. Some have rigid cell walls, while others have more flexible cell membranes. Their methods of movement include passive drifting, swimming with flagella, swimming with cilia and creeping along with pseudopods. Even certain very basic criteria that have been used to define the group, such as the presence of nuclei and mitochondria, either don't exist or take on bizarre forms in some protists.

Cross-Kingdom Characteristics

Scientists have tried to classify the organisms within the protists as either plant-like, fungus-like, or animal-like. However, genetic testing and close examination have revealed that these categories often don't hold up, either. For example, Euglena have characteristics of both plant-like and animal-like protists. Euglena have chloroplasts like plants, in the sense that the chloroplasts allow them to gain energy from the sun through photosynthesis. At the same time, they have a tail or flagella which they use to swim, making them mobile, a very animal-like characteristic. Many other protists also have characteristics that make it difficult to justify keeping them all in a single group or subgroup.

8 0
3 years ago
In pointers dogs, the Punnett square for tail length is below (gene A). Use the Punnett Square below to determine the possible o
kvv77 [185]

Answer:

a) Autosomal dominant inheritance ⇒ The presence of at least one A1 allele is enough to express Bow-tie tails.

b) Autosomal recessive inheritance ⇒ The presence of at least one recessive allele A2 is enough for individuals to survive.

Explanation:

Due to technical problems, you will find the complete explanation in the attached files.

Download pdf
5 0
3 years ago
Which of the following is a process that is part of the water cycle?
svet-max [94.6K]
Water from clouds evaporates into the ocean.

Answer: A
6 0
3 years ago
What are the "twisty columns" and "rungs" in the DNA?
kolezko [41]
The twisty columns of the double helix are phosphate and sugar(deoxyribose), which are bonded by a covalent bond. Each nucleotide consists of one of each, and also a nitrogen base. The rungs are nitrogen bases, each nucleotide has one base, and one side of the ladder bonds to the other side of the ladder through hydrogen bonding of complementary base pairs. The base pairs of DNA are A-T and C-G
7 0
3 years ago
Read 2 more answers
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