The light brown substance obtained from the outer layer of the bark of the cork oak
The Adipose tissue mainly works as storing energy into fat but it also works as an insulating layer that allows less heat to escape the body, as well as offer protection to major organs.
Answer:
Plasma.
Explanation:
Plasma is the liquid component or constituent of the blood after the removal of all the cells and platelets. It is composed of 90% water, coagulation factors like antibodies, electrolytes, proteins , lipids e.t.c which help to maintain the blood PH and osmotic balance of the blood during the process of giving viscosity to the blood. The plasma comprises of 55% of the blood.
The given question is incomplete, the complete question is:
A student carries out PCR using the following steps: Step 1: 94°C for 1 minute Step 2: 60°C for 30 seconds Step 3: 72°C for 30 seconds After subjecting his PCR reaction to gel electrophoresis, the student sees no PCR product on the gel. What error(s) might he have made? (Select all that apply.) a. He carried out step 1 at too low a temperature. b. He carried out step 1 at too high a temperature. c. He carried out step 2 at too low a temperature. d. He carried out step 2 at too high a temperature. e. He carried out step 3 for too short a time.
Answer:
The correct answers are options d and e, that is, the student might have carried the step 2 at too high temperature, and he would have carried the step 3 for a brief duration.
Explanation:
In the PCR, step 1 is termed as the denaturation step and it generally takes place at 94 or 95 degrees Celsius. Thus, option a and b are incorrect. In step 2 of the PCR reaction, the combination of primers takes place with the template, this step is known as the annealing step. The temperatures in the annealing step are important for PCR, if the temperature of annealing is too much, it can lead to no product formation, and if it is too less than the formation of products would be non-specific.
In step 3 of the PCR reaction, the temperature is extended and the Taq polymerase enzyme is used to perform the reaction, which supplements nucleotides to the newly developed stand, at the frequency of 1 kilobase pair per minute. If the product of PCR is one kilobase or more, then a 30 seconds extension time would be not enough and the product would fail to appear. Hence, the correct options would be d and e.