Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
The answer is 'Cambrian' explosion. This took place approximately 541 million years ago. 'Explosion' refers to the evolution of a large range of new major animal phyla. Scientists know this by the large number of diverse fossils from this period. Prior to this period, organisms were single-celled, but over the Cambrian Explosion, lasting about 80 million years, life forms diversified rapidly to resemble the life forms existing today.
Interrupt, rupture
contradict, verdict
respect, import
deport, inspect, transport
This is what I got. I hope this helps :)
An ideal gas is a theoretical gas in which inter molecular forces do not exist among the molecules and the molecules do not occupy any space. A gas that does not obey ideal gas law are described as non ideal in behaviour. Gases that are most susceptible to non ideal behaviour are those that have attractive and repulsive forces among their molecules and whose particles have volume. Gases that exhibit non ideal behaviour has compressiblity factor ratio that deviate from 1.
Answer:
Phagocytic defense in the normal lung is shared principally by two kinds of cells - alveolar macrophages that reside on the air surface and roam the alveoli and PMNs that circulate in the intravascular space or are stored transiently in areas adjacent to the capillary-alveolar interface (marginated in capillaries)
Explanation:
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