Question

The triple sugar-iron (TSI) agar test surveys the use of different carbohydrate fermentation pathways and the presence or absence of hydrogen sulfide production to differentiate among the different groups of what large and diverse group of bacteria?

Answer 1

Answer:

The group is called  Enterobacteriaceae.

Explanation:

When lactose (or sucrose) is fermented, a large amount of acid is produced, which turns the phenol red indicator yellow both in bottom and in the slant (Escherichia, Klebsiella, Enterobacter).

If lactose is not fermented but the small amount of glucose is, the oxygen-deficient bottom will be yellow (remember that butt has comparatively more glucose than slant i.e. more media more glucose), but on the slant the acid produced (less acid produces in slant as media in slant is less) will be oxidized to carbon dioxide and water by the organism and the slant will be red (alkaline or neutral pH) (Shigella, Serratia, Salmonella, Proteus).

If no sugar is fermented, both the butt and the slant will be red (Pseudomonas. )

if H2S is produced, the black color of ferrous sulfide is seen (Salmonella, Proteus).