Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Breed "pure" mice with known genotypes that exhibit specific fur and eye colors, and learn how traits are passed on via dominant and recessive genes. Mice can be stored in cages for future breeding, and the statistics of fur and eye color are reported every time a pair of mice breed.
Answer:
A student has been studying electrical circuits in class. The student suggests that replacing a wire with rubber tubing will stop the current from flowing through a circuit. Which term best describes this idea?
Hypothesis
Explanation:
From the analogy, it is expedient to know that the student assertion about the electrical circuits was an hypothesis because it gives room for further investigation by other scholars.
Fungi are responsible for what common human ailment athlete's foot infections
According to a source the answer is <span><u>Cyanobacteria were the first organisms to produce oxygen in water, which then escaped into the atmosphere.</u>
These cyanobacteria species were the first ones to "produce" oxygen as a by-product of the photosynthetic mechanisms which enables organisms to make their own food. </span>
