Answer:
Use a step wise process
Explanation:
In order to find the presence of the recombinant protein:
- Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.
- Extract the plasmid from both strains.
- Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control.
- Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis.
- Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.
Enzymes are catalysts that speed up chemical reactions in the human body.
<h3>What do you mean by Catalysts?</h3>
Catalysts may be defined as those substances that enhance the rate of chemical reactions without being consumed in them.
Enzymes are also considered as catalysts in the body of living organisms. Basically, enzymes are proteins that assist in the speeding up of any chemical reaction in living entities.
Therefore, enzymes are catalysts that speed up chemical reactions in the human body.
To learn more about Catalysts, refer to the link:
brainly.com/question/16781912
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Fossil is the remains of plants and animals.
