Fresh water is any body of water with a low salt concentration. Organisms in fresh water various challenges which includes; Osmotic concentration such that fresh water is permanently dilute and most cells cannot function at this concentration of ions. Varying temperatures, variation in oxygen levels and reproduction and life cycle due to permanence of habitat and physiological difficulty of regulating osmotic concentration in larvae and juveniles.
Some abiotic factors that can limit a population are space and weather these are called limiting factors (and how much water is provided)
Mitosis creates two identical daughter cells that each contain the same number of chromosomes as their parent cell. ... These new combinations result from the exchange of DNA between paired chromosomes. Such exchange means that the gametes produced through meiosis exhibit an amazing range of genetic variation.
https://www.nature.com/scitable/topicpage/replication-and-distribution-of-dna-during-meiosis-6524853
