Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
Answer:
Eukaryotic cells are plants, animals, protists, fungi. Their genetic material is organized in chromosomes. Golgi apparatus, Mitochondria, Ribosomes, Nucleus are parts of Eukaryotic Cells.
Animals such as cats and dogs have eukaryotic cells.
Plants such as apple trees have eukaryotic cells.
Fungi such as mushrooms have eukaryotic cells.
Protists such as amoeba and paramecium have eukaryotic cells.
Insects have eukaryotic cells.
Humans are composed entirely of eukaryotic cells. Related Links: Examples.
Explanation:
Bacterial strains, especially Actinomycetes have been used in biotech production and drug discovery for years. Genetic methods now open the field of combinatorial biosynthesis that has improved impressingly in the past couple of years. Also, the productivity of yeast and other fungi in a variety of different processes has improved significantly since genetic methods have been introduced. In addition, a number of recent works considerably widens the potential of plant biotechnology. This review covers examples describing the use of prokaryotic cells and plant cells in biotech production.
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The process of turning water into ice is known as Condensation.
Condensation converts a substance from a gaseous state to a liquid form, frequently with a reduction in volume. It happens when a gas or vapour comes into touch with a surface that radiates heat, causing the gas or vapour to cool and solidify. Ice is the solidified version of water, which is a liquid. Condensation is the process by which water changes from the liquid phase (which is water) to the solid phase (which is ice).
Hence, The process of turning water into ice is known as Condensation.
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Answer:
Fenestrated capillaries
Explanation:
Fenestrated capillaries are found in some tissues where there is extensive molecular exchange with the blood such as the small intestine, endocrine glands and the kidney.