I think It purifies the soluble GFP in the supernatant by passing over the supernatant over an HIC columnin a highly salty buffer.
Answer:
The sine or sin is a trigonometric function of an angle. The sine of an acute angle is defined in the context of a right triangle: for the specified angle, it is the ratio of the length of the side that is opposite that angle, to the length of the longest side of the triangle (the hypotenuse).
Hope this helps you!
Explanation:
The answer is : linea aspera
We feel hotter in summer than in winter because the temperature is generally higher than in winter.
<h3>
What are seasons?</h3>
There are three seasons on earth namely winter, summer, and rainy season. In the winter season, the temperature is cold and during summer the temperature is higher.
Timing and characteristic of season depend upon the location of the earth. The time of a year region experience season depends on it is northern hemisphere or southern hemisphere.
The southern hemispheres experiences winter while northern hemisphere experiences summer. The cycle of seasons is caused by earth's tilted towards the earth. The planet rotates around an axis.
Therefore, We feel hotter in summer than in winter because the temperature is generally higher than in winter.
To learn more about seasons, refer to the link:
brainly.com/question/19009677
#SPJ2
The atomic number of an isotope is the number of protons it contains, whereas the mass number is the total mass of the nucleus, which is the combined number of protons and neutrons in the nucleus. To find the number of neutrons, you must therefore subtract the atomic number of the isotope from the mass number of the isotope.
<span>In the above example, the atomic number is 8 and the mass number is 18. This isotope is known as O18. The number of neutrons in the isotope is: Mass number - Atomic number = Number of neutrons
</span>
