Answer: 5 prime cap
Shortly after RNA polymerase begins to transcribe a pre-mRNA, a 5 prime cap is added.
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The answer is A.
You can remember this by thinking that since there was no change in the amino acid sequence, the mutation is silent in the fact that it didn't cause any trouble in the amino acid sequence.
If the paramecium was not able to contract its contractile vacuole, it would be in danger of bursting. The cell would not be able to hold too much water. This would happen faster if the paramecium was in water with a low salt concentration because there is more water and less salt, so the water would accumulate faster.