in an experiment you found two proteins(protein x and protein z)with similar size and sequence homology.Two domains of both prot
eins are nearly identical,but each possesses adifferent sequence in another domai .which antibody would be the best selection for you to identify the expression of protein zbur not protein x using a western blot?
Monoclonal antibodies are antibodies that are made by identical immune cells which are all clones of a unique parent cell.
Monoclonal antibodies can be isolated such that they will be able to only recognize a unique epitope, this makes them to be suitable for determining the expression one or two identical sequenced proteins. Monoclonal antibodies technique is one of the most effective chromatography techniques.
Polyclonal antibodies contain different epitopes on a protein, hence suitable for protein identification but not suitable for determining the expression of one or two identically sequenced proteins.
monoclonal antibody is a protein manufactured by the body's immune system to safeguard the body against antigens . Antigens such as bacteria, viruses, parasites, fungi, and other substances that the immune system classify as foreign. Few times the body mistakenly classifies normal tissues as antigens and creates antibodies to counter them. This is the major cause of autoimmune conditions e.g. multiple sclerosis and rheumatoid arthritis.
