Explanation:
A similar question was asked online, here is the answer it gave:
'“Negative control” is a treatment that by definition is expected not to have any effect (neither positive effect, nor negative effect). “Positive control” is treatment with a well-known chemical that is known to produce the expected effect with the assay that you are studying. Application of an antagonist is not a negative control in your case. “Negative control” is condition that should be treated with the same solutions or buffers as your “treatment” condition, with the only difference that instead of the chemical that you investigate you should add just the solvent that was used to dissolve you chemical in the respective final concentration that you have in the “experimental treatment” condition. For example if your chemical is dissolved in DMSO – than the correct negative control will be to add to the medium/buffer just DMSO in the same final concentration that you reach with your “treatment” condition. One of the reasons of using such negative control is to verify that the solvent is having no effect in your assay. Note that among all treatment conditions (“negative control”, “positive control”, “experimental treatment you are investigating”) the volumes and the composition of the treatments that you are doing should be uniform: always treat with the same volume of medium or buffer, always containing the same concentration of the used solvent (e.g., DMSO). The only difference should be the presence or absence of the defined compound-treatments (agonist, antagonist, the chemical for the experimental investigation etc.).'
My best advice is to use the textbook you have, or use examples of a negative control when testing organic compounds because you have to find something that you can assign, like a worm in a box of dirt, the worm could have enough food to survive, so that is your negative control, but when it comes to finding the best, that would have to rely on something within the parameters of being self sufficient like a plant getting its energy from photosynthesis, etc.
Atanasov, Atanas. (2013). Re: Positive control and negative control. Retrieved from: https://www.researchgate.net/post/Positive_control_and_negative_control/515968f2d039b1fe50000025/citation/download.
Answer:
genetics, or different genes I think
Answer:
30% Thymine
Explanation:
If 30% of Adenine is present, it would be reasonable to assume that 30% of Thymine will be present, as the ratio between the two complementary nitrogenous bases will be roughly equal.
Therefore, that leaves us with 40% between Cytosine and Guanine. Since the ratio between them would be equal, it gives us 20% of Cytosine and 20% of Guanine.
<u>Therefore:</u>
30% of A + 30% of T + 20% of C + 20% of G = 100% DNA
Hi,
Recombinant DNA technology is the process in which a target gene of interest is replicated by inserting it into a vector and creating a recombinant DNA. This recombinant DNA is then returned into an expression system where it is replicated several times to give rise to multiple copies of our desired gene or that gene product.
One of the very important role for the execution of this technology is performed by Restriction endonuclease enzymes. These are the enzymes that have ability to cut the DNA fragment at specific sequence into fragments of different lengths called restriction fragments.
- They play a very important role in recombinant DNA technology:
- They can map the location of specific restriction sites where the target sequence is identified and cleaved.
- They cut down the DNA at specific sites called restriction sites. The host DNA in which foreign gene or target gene is inserted is also cut with the same restriction enzymes. So in the recombinant DNA technology, restriction enzymes act like a molecular scissor which identify and cut certain DNA sequences as per or requirement.
- The target sequence can be cut from multiple places by restriction enzymes since our target sequence can be present multiple times in the DNA of organism.
- When restriction enzymes cut the DNA, they leave sticky or blunt ends at that site in such a way that these ends are able to bind with the complementary bases of vector DNA when introduced.
- Then another enzyme DNA ligase seals the ends of target DNA and vector DNA making a whole recombinant DNA that is ready to be cloned and progress further steps of recombinant DNA technology.
<em>You can see attached image for better understanding.</em>
Hope it help!
