Answer:Chromatography is actually a way of separating out a mixture of chemicals, which are in liquid or gas form, by letting them flow slowly past another substance, which is either a solid or a liquid. It consist of a stationary phase and a mobile phase.
Explanation: All chromatographic systems rely on the fact that a substance placed in contact with two unmixable phases, one movable phase and one stationary phase, will equilibrate between them. A selectivity (or separation) factor (α) is used to 'chemically' distinguish between sample components. It is usually measured as a ratio of the retention (capacity) factors (k) of the two peaks in question and can be visualized as the distance between the apices of the two peaks. reproducible fraction will partition into each phase, depending on the relative affinity of the substance for each phase. A substance which has affinity for the moving or mobile phase will be moved rapidly through the system. A material which has a stronger affinity for the stationary phase, on the other hand, will spend more time immobilized in that phase, and will take a longer time to pass through the system. Therefore, it will be separated from the first substance. By definition, chromatography is a separation technique in which a sample is equilibrated between a mobile and a stationary phase. A theoritical plate or tray is used to produces the best possible difference between the liquid and vapour phases in equilibrium with it
Chromatographic separations are best done with a small amount of analyte (substance to be separated during analysis), which keeps either phase from becoming saturated with analyte, so that the concentrations in the two phases are directly proportional. Overloading the column with sample causes one of the phases to become saturated, leading to a loss of column efficiency, and poorly shaped peak profiles.
The retention volume in chromatographic separation (Vr) is the volume of the mobile phase required to carry the solute through the column to elution, is related to the column flow (Fc) and the retention time (tr). Likewise, the volume of the mobile phase(Vm), is related to the flow and the time the void volume takes to pass through the column.
Band broadening using the kinetic model is a phenomenon that reduces the efficiency of the separation being carried out, leading to poor resolution and chromatographic performance. This is problematical in terms of both the quality of the separation obtained and the accuracy with which sample components can be quantified.The wider band results in a dilution effect that produces a decrease in peak height accompanied by a loss in sensitivity and resolution. The eddy dispersion, accounts for the source of band broadening related to any flow unevenness in the column.
The question is incomplete, however, the statements associated with this question is given in the comments and here as well:
Neither facilitated diffusion nor osmosis requires cell energy.
Diffusion of gases and other small molecules requires no energy on the part of the cell.
Active transport requires cell energy and osmosis doesn't.
Both endocytosis and active transport require cell energy.
Answer:
The correct answer is - Active transport requires cell energy and osmosis doesn't.
Explanation:
Osmosis is an example of passive transport as it does not require energy to facilitate the movement of solvent In the process of osmosis,. It moves from high concentration to low concentration through the semipermeable membrane which is along the gradient so no requirement of energy.
In the case of Active transport, it requires energy to facilitate the movement of transport as it is the movement of a substance from low concentration to a high concentration area that is against the concentration gradient.
Answer: Like with fingerprints, no two people have the same brain anatomy, a study has shown. This uniqueness is the result of a combination of genetic factors and individual life experiences.
Explanation: An example is the heart's circulatory system, where the vessel supplying blood to the posterior part, known as posterior descendant artery, stems from the right coronary artery in 80-85% of the cases, and from the left coronary artery in the remaining 15-20%. This is known as either right of left dominance
<span>The cell nucleus contains the majority of the cell's genetic material in the form of multiple linear DNA molecules organized into structures called chromosomes.</span>
Answer:
3
Explanation:
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