1) What sequence of nucleotides does the guide RNA need to include, to kill the bacterium is the answer.
2). The action of the Cas-9 protein.
Explanation:
CRISPR is a genetic engineering technology used to detect and destroy DNA of the bacteriophage which have infected the bacterial cell previously.
The CRISPR are the sequences of bacteriophage DNA found in genome of the bacteria when bacteriophage has infected it before.
It alters the DNA sequences and modify the function of gene is bacteria.
Cas-9 is an endonuclease which cuts the DNA identified by or complementary to CRISPR.
This system makes bacteria anti viral.
It is an example of acquired immunity.
