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GrogVix [38]
3 years ago
9

Which statement regarding the light reactions is true? A Photoexcited electrons pass from photosystem I to photosystem II.B Gluc

ose is a product of the light reactions.C Electrons are supplied to chlorophyll P680 as it reduces the primary electron acceptor.D Electrons "falling" down the electron transport chain are deposited on ADP to form ATP.
Biology
1 answer:
Annette [7]3 years ago
3 0

The correct answer is: C Electrons are supplied to chlorophyll P680 as it reduces the primary electron acceptor

Light dependent reactions are part of the photosynthesis and they occur in the thylakoid membranes of chloroplasts. During this step of photosynthesis, molecule ATP and the reduced electron carrier NADPH are produced that can be used for the further reactions. The main role in light dependent reaction are photosystems (complexes of proteins and light-absorbing molecules-pigments):  

Photosystem I (PSI)- pair of chlorophyll P700

Photosystem II (PSII) - pair of chlorophyll P680

Energy is transferred from pigment to pigment in PSII to P680, where an electron is boosted to a high energy level. That high-energy electron is then passed to an acceptor molecule and replaced with an electron from water.  

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tresset_1 [31]

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(1) a mutation in the coding region, resulting in an inactive protein

To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.

To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.

(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.

To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.

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