1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Stella [2.4K]
3 years ago
14

In colorectal cancer, some tumor suppressor genes are inactive. This is an important factor resulting in uncontrolled cell divis

ion. Two of the possible explanations for the inactive genes are: (1) a mutation in the coding region, resulting in an inactive protein, and (2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription. How would you investigate each of these possibilities?
Biology
1 answer:
tresset_1 [31]3 years ago
3 0

Answer/Explanation:

(1) a mutation in the coding region, resulting in an inactive protein

To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.

To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.

(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.

To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.

To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR

You might be interested in
What is a waste product of photosynthesis that organisms need for cellular respiration
Aloiza [94]

Answer:

Oxygen

Explanation:

A waste product of photosynthesis organisms need for cellular respiration is oxygen. We breathe out carbon dioxide and plants use carbon dioxide during photosynthesis and in return they give us oxygen which is their waste product.

8 0
3 years ago
In heterotrophs, energy for the life processes comes from the chemical energy stored in the bonds of
Delicious77 [7]
Humans get there energy primarily from glucose I believe. We break down glucose to get ATP (Adenosine Triphosphate) which is then used to supply energy to our cells in order to function. 
7 0
3 years ago
Why do new species evolve more rapidly after a mass extinction?
Nuetrik [128]
Because they have no competitors in any echological niche
3 0
3 years ago
Read 2 more answers
The community level of ecological organization include all other levels true or false
Charra [1.4K]
<h2>its true</h2><h2>the ecological system of organization is a gigantic scale of all sorts of sh*tt</h2>
3 0
3 years ago
Which type of marine ecosystem do you think supports the least life
Kobotan [32]
Probably the intertidal zone, as I reason the tides would affect life dramatically. High and low tide would affect land and ocean creatures in this zone too often where as the coastal and open oceans have constant properties.
5 0
3 years ago
Read 2 more answers
Other questions:
  • Why is having an extra chromosome 21 tolerable to the point that someone with this condition can survive to maturity?
    11·1 answer
  • How is protein synthesis different from DNA replication?
    11·1 answer
  • Biology help! I will give brainiest, and I will report you if you give a cruddy answer.
    6·2 answers
  • What role do roots play in photosynthesis?
    7·1 answer
  • What agent of erosion creates a cirque?
    9·1 answer
  • A(n) is a sequence of DNA nucleotides that codes for a specific protein or RNA molecule
    13·2 answers
  • Both aerobic respiration and anaerobic respiration utilize glycolysis, Krebs cycle, and an electron transport system, but in ___
    5·2 answers
  • Which term describes the tendency of an organism to regulate internal conditions for maintaining good health?
    7·2 answers
  • How does the respiratory system interact with the circulatory system?
    11·1 answer
  • What is a specialized cell? Give an example.<br> What does it mean when cells differentiate?
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!