Question

A scientist has a very small sample of DNA. To analyze the DNA, which would be the MOST USEFUL first step for the scientist to follow?
A. Use restriction enzymes to make multiple copies of the DNA.
B. Use the polymerase chain reaction (PCR) to make multiple copies of the DNA.
C. Transfer the DNA into bacteria, and allow the bacteria to be replicated many times.
D. Use restriction enzymes to cut the DNA into many small pieces.

Answer 1

Answer:

B. Use the polymerase chain reaction (PCR) to make multiple copies of the DNA.

Explanation:

The scientist is to analyze a small sample of DNA, the most useful step to start this would be first the use of polymerase chain reaction to make various copies of that DNA sample. PCR is essential and quick process that gives a lot of copies within few hours. It uses the sample or target DNA, according to which the primers are designed. Taq polymerase and DNA polymerase are also used in the process, it consist of various cycles in which copies are made. There are three basic steps 'Denaturation', 'annealing', and 'extension'.