<span>The most crucial principle of ELISA (enzyme-linked immunosorbent assay) is a highly specific antibody-antigen interaction.</span>
ELISA is a biochemical technique used to detect the presence of an antibody or an antigen in the biological sample. Simply described, in an ELISA, an antigen is immobilized on a solid surface and then a specific antibody is applied over the surface so that it can bind to the antigen. The antibody is usually linked to an enzyme, and in the final step, a substrate for that enzyme is added. The enzyme can convert it to some detectable signal, most commonly a color change. <span>Medical usage of ELISA is in the diagnosis of HIV infection, pregnancy tests, measurement of cytokines…</span>
Answer:
A punnet square can be described as a diagram which is made to check the outcomes of a cross.
To check the probability of the offsprings of a cross between homozygous dominant thumb shape parent with a homozygous recessive thumb shape parent, let's draw a punnet square:
t t
T Tt Tt
T Tt Tt
The results of the punnet square depict that:
Genotype: All of the offsprings will be heterozygous for the thumb shape trait. Tt will be the genotype.
Phenotype: All of the offsprings will show the thumb shape like the thumb shape of the dominant parent.
Answer:
The results show that the increase in the substrate concentration is increasing the rate of the reaction. We can see that the reaction rate has increased to 2.88mM/min. After this even if we increase the substrate concentration
The answer is letter B has commercial uses such as Alcohol production and bread making.
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