Answer:
soil temperature soil nutrients soil ph
Answer:
Short answer is primers are partially complementary.
Explanation:
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3'
Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3'
When we write reverse primer 5' to 3' we can easily see that
3'-TTGGCCAATGG---5' is complementary to the forward primers'
5'---AACCGGTTACC-3' sequence. So instead of binding to the template DNA these primers might bind each other resulting with reduction of efficiency of DNA amplification.
Scientists who do this are doing what is considered to be and called cloning. Cloning is exactly this - taking cells from one organism and trying to produce and recreate an identical (from a genetic perspective) organism.
This can be done in a routinely fashion but there are serious ethical considerations nowadays.
Answer:
You need to quickly determine if any bacteria are growing in your new broth media. The fastest way to check for growth would be to use: a chemostat.
Explanation:
