Answer:
The correct answer is option D.
Explanation:
In the presence of SDS single band appeared, while in the absence of SDS two bands appeared. SDS or sodium dodecyl sulfate refers to an anionic detergent, which combines with amino acid side chains providing the protein a net negative charge. It dissociates the non-covalent bonds.
In SDS-PAGE, the separation of proteins takes place on the basis of their molecular weight. Options A and B are incorrect as only in the presence of SDS, the separation of protein subunits takes place. Option C is also incorrect as a protein containing distinct molecular weight cannot show single band.
Option D is correct as the presence of SDS supplements a bunch of negative charges to the protein, thus, charge is not the factor. Therefore, the proteins are distinguished on the basis of the molecular weight. Thus, identical molecular weight demonstrates a single band. In the non-presence of SDS, charge performs a function along with the molecular weight, therefore, two bands appear.
This is a issue of morality and ethics. So the answer to the first question depends on the perspective of the person. Personally I don't agree with performance enhancing drug use but I do think some of the expectations of athletes who perform in Olympic events increase the pressure to have to use them. So do I think people who use these drugs should be elected into the Hall of Fame. That is hard to answer. But I do not think using drugs takes away all of the achievement an athlete has made. It just proves that the athlete couldn't achieve the rigid sport performance without manipulation. But it doesn't mean they haven't worked hard or trained.
I do think rules should be made for athletes. These rules would definitely weave through most of the athletes we see doing these out of the world sport records every day. That is because many of them have been proven to have used these performance enhancing drugs.
Please vote my answer brainliest. thanks!
I’m pretty sure is the 3rd one
