Answer: a conversation or oral communication to get technical
Explanation:
Ozone filters the UV radiation (emitting by Sun). By doing that, it keeps our environment safe & healthy.
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Agency problem
Agency problem also known as agency costs occurs in a two-party relationship (principal/agent) where the agent is expected to act or make decisions for the good of the principal.
For example in a corporate the relationship between the management and shareholders. The management is expected to make decisions that will maximize shareholders interest. The problem arises when the two parties have different interests. In the example above the manager may opt to make his own wealth and not act in the company’s best interest which could be maximizing company’s market value.
Examples of agency relationship in finance
Managers/stockholders
Managers/Creditors
Causes of conflicts between managers and stockholders may include;
Remuneration - low remunerations or fixed salaries despite increased profit margins.
Differences in risk profile- stockholders may prefer high-risk return investments contrary to the managers. When high-risk investment go bad the manager risks job loss
Manipulation of accounting systems- to reflect high profits.
Unnecessary perks management award themselves.
Solution to these problems include threat for firing in case of poor performance, shareholders may also threaten to sell the company, remuneration based on performance, incurring agency costs-these are costs incurred while hiring external auditors, setting a control system, legal costs for employment letters and contracts.
Agency problem may be reduced by motivating the manager to act for the companies best interest by offering incentives
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Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.