Ans. (B). They use different nucleotide bases.
DNA replication involves synthesis of DNA molecules, while transcription is a process that involves synthesis of RNA molecules. In both processes, DNA acts as template and provides information for synthesis of new daughter DNA or RNA strand.
Process of replication and transcription differs from each other as each process uses different nucleotide bases. This is because, nucleotide bases in DNA include adenine, guanine, cytosine, and thymine, while in RNA uracil is present instead of thymine along with three similar bases.
Thus, the correct answer is option (B).