Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Its B.cell wall
ribosomes are used to synthesis proteins and other components required for cells
endoplamsic reticulum is where the ribosomes are its a structure that helps to prepare the components and to bring them to the golgi body
the cell wall is only present in plants (cellulose) and fungi (chitin) and it helps plants to regulate their water intake physically
the cytoplasmis present in all cells as the medium which organelles are suspended in and in contact with
hope that helps <span />
30% and 70% dont usally suffer from excess bleeding
Answer:
A
Explanation:
Viruses don't contain organelles.
