What is the most basic structure block for all life
1 answer:
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Answer:
b. aaBb x AAbb
Explanation:
According to the information in this question, the trait for magic ability is controlled by two genes, each comprising of two alleles i.e. A,a and B,b. However, to be able to perform magic, a person would need to carry at least one copy of the dominant allele (A and B) on each of the two loci.
Hermione Granger has magical ability but neither one of her parents does. This means that although Hermione can perform magic, none of her parents can. Genetically, it means that Hermione posseses at least one copy of the dominant allele on each of the two loci i.e. her genotype is A_B_.
This is only possible in Hermione if her parents transferred a dominant allele at each loci to her. This means that the possible genotypes for Hermione Granger’s parents are aaBb (no magical ability) x AAbb (no magical ability). See the punnet square attached where the probability of producing offsprings with magical ability is 1/2 or 50%.
1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein.
2.Let sit at 55 degrees for at least 1 hour. Function: This temperature is optimal for enzyme reaction; if too high will degrade DNAase.
3.Add 1/10 volume of Kac (potassium acetate) (5 M; pH 5.6) (in this example, 20 ul but remember the 1/10 proportion as a general rule). . Mix well and place on ice for 10 minutes. Function: precipitates membrane, C, H, O, lipids, proteins, and monomers.
4.Add 200 ml of phenol saturated with 10mM Tris. ( IN HOOD) Function: removes nonpolar proteins, lipid residue.
5.Cap tube: mix well by inverting tube.
6.Spin 5 minutes in microfuge.
Function: separates aqueous (top) and phenol (bottom) layers 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube.
8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA.
9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
10.Remove upper aqueous layer (CONTAINS DNA) to a new tube.
11.Add 500 ul of EtOH and cap tube (again, 500 in this example; as a proportion use twice the total amount). Function: precipitates DNA; DNA is insoluble in ethanol.
12.Cap tube; mix well by inverting tube several times.
13.Spin for 5 minutes in microfuge. 13000 rpm. (10 min. at 6000.) Function: collects DNA in bottom of the tube.
2.Let sit at 55 degrees for at least 1 hour. Function: This temperature is optimal for enzyme reaction; if too high will degrade DNAase.
3.Add 1/10 volume of Kac (potassium acetate) (5 M; pH 5.6) (in this example, 20 ul but remember the 1/10 proportion as a general rule). . Mix well and place on ice for 10 minutes. Function: precipitates membrane, C, H, O, lipids, proteins, and monomers.
4.Add 200 ml of phenol saturated with 10mM Tris. ( IN HOOD) Function: removes nonpolar proteins, lipid residue.
5.Cap tube: mix well by inverting tube.
6.Spin 5 minutes in microfuge.
Function: separates aqueous (top) and phenol (bottom) layers 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube.
8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA.
9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
10.Remove upper aqueous layer (CONTAINS DNA) to a new tube.
11.Add 500 ul of EtOH and cap tube (again, 500 in this example; as a proportion use twice the total amount). Function: precipitates DNA; DNA is insoluble in ethanol.
12.Cap tube; mix well by inverting tube several times.
13.Spin for 5 minutes in microfuge. 13000 rpm. (10 min. at 6000.) Function: collects DNA in bottom of the tube.