Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
<span>Narrow junctions and desmosomes have different structures. Narrow junctions are made of a protein arranged inside the membrane. There are many intersecting lines that cause the cells to be sealed and together. Desmosomes contain the protein cadherin. There are cadherins in each cell and they join at this point sealing the cells together, the tight junctions have the function of preventing the materials that circulate between the cells. The desmosomes provide binding sites for the cells to be attached.</span>
Answer:
An intermediate is a species which appears in the mechanism of a reaction, but not in the overall balanced equation. Examples: Amphibian/land vertebrate (Pederpes)- Intermediate form between primary aquatic Upper Devonian amphibians and early tetrapods. Lizard/snake (Pachyrhachis)—Intermediate form of snakes and an extinct lizard-like reptile. It was a primitive snake with limbs.
Explanation:
Answer:182.25 joules
Explanation:
Mass=0.18kg
Velocity=45m/s
Kinetic energy=(mass x(velocity)^2)➗2
Kinetic energy=(0.18 x 45^2) ➗ 2
Kinetic energy=(0.18x45x45) ➗ 2
Kinetic energy=364.5 ➗ 2
Kinetic energy=182.25
Kinetic energy=182.25 joules
