What would be the consequence of treating a cloning vector, before ligation, with calf intestinal phosphatase? What would be the

Question

4 years ago

7

What would be the consequence of treating a cloning vector, before ligation, with calf intestinal phosphatase? What would be the

consequence of treating a cloning vector, before ligation, with calf intestinal phosphatase? It would ligate the ends of the plasmids. It would prevent the plasmid's DNA from reversing polarity during ligation. It would prevent the ends of the plasmids from being ligated. It would reverse the polarity of plasmids' DNA

Biology

1 answer:

OlgaM077 [116] · Answer 1 · 2022-02-02T11:59:00+03:00

Answer:

It would prevent the ends of the plasmids from being ligated.

Explanation:

The function of this enzyme is that it catalyzes dephosphorylation, removing phosphate groups from the 5' ends of the the DNA strands. It particularly functions in preventing the re-ligation of a plasmid DNA that has been linearized by this method such that other DNA fragments can be ligated into the vector. It is particularly used in cloning.