It can be concluded that the cells on the slide are acid-fast bacteria if red colored spots are seen on the slid containing acid fast stain.
Further explanation:
The acid-fast organisms such as mycobacteria can be differentiated from the other bacteria by the help of acid-fast staining. These acid-fast bacteria show a very high concentration of mycolic acid in the cell walls. The two stations that are used in this type of staining technique are carbolfuchsin and methylene blue.
The acid-fast organisms retain the red color of carbolfuchsin even after the decolorizing agent is added to it. The non-acid-fast organisms do not retain the red color of carbon fusion and thus, are counterstained by methylene blue.
All the gram-positive bacteria are acid-fast bacteria. In addition to the peptidoglycan layer, the acid-fast bacteria have a cell wall containing large amounts of glycolipids (mycolic acids). <em>Mycobacterium tuberculosis and Mycobacterium leprae are the two examples of acid-fast bacteria. </em>
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Answer Details:
Grade: High School
Subject: Biology
Topic: Acid-fast staining
Keywords:
Acid-fast, bacteria, carbolfuchsin, counterstain, methylene blue, decolorising agent, cell wall, glycolipids, mycolic acids, mycobacterium