Question

Total nucleic acids are extracted from a culture of cells and are then mixed with resin beads to which the polynucleotide 5'-TTTTTTTTTTTTTTTTTTTTT-3' has been covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyze the cellular nucleic acids that have stuck to the beads, which of the following molecules do you expect to be most abundant?A. DNA.
B. tRNA.
C. rRNA. (it is not this one, I put this and it was wrong.)
D. mRNA.
E. Primary transcript RNA.

Answer 1

Answer:

D. mRNA.

Explanation:

Polyadenylation is defined as the addition of poly (A) to messenger RNA. This tail is made up of several adenosine monophosphates (adenine bases). In eukaryotes, polyadenylation is part of the process in which messenger RNA is formed for subsequent translation. Therefore, it is part of the process of gene expression.

Answer 2

Answer: D mRNA

Explanation:

mRNA (Messenger RNA) is a single-stranded RNA molecule that corresponds to the genetic sequence of a gene and is read by the ribosome in the process of producing a protein and it carries the genetic information copied from DNA in the form of a series of three-base code “words,” each of which specifies a particular amino acid.mRNA is the most abundant because it is the only type of RNA that is polyadenylated and the poly (A) tail would be able to base pair with the strands of poly(T) on the beads and thus stick to them. DNA would not be found in the sample, as the poly (A) tail is not encoded int he DNA and long runs of T are rare in DNA.