The definition of transformation in bacteria is described by the first statement: transformation is the assimilation of external dna into the bacterial cell. In a more elaborate sense, transformation is described by the altering of the cell as a result of the uptake or intentional incorporation of dna from an external source. It is one of the three processes for horizontal gene transfer. The other two are transduction (infection of a phage), and conjugation (transfer of dna between two bacterial cells that are directly in contact).
50% of their offsprings will have the recessive trait because if you use Punnett square to analyze the data you will see that only 50% of their children will have Tt as their allele
Peristalsis helps in storing food, breaking down food down and mixing it with juices secreted by stomach lining. Peristalsis in stomach helps in partial digestion of food called chyme.
This can select for resistant mosquitoes. After a couple of years, the population is entirely resistant to the insecticides you used. The insecticides may also kill off the beneficial insects (like pollinators e.g. bees) or the predator insects that ate the mosquitoes. Bioaccumulation and biomagnification in the food chain can lead to unintended deaths of high-level predators, e.g. DDT leading to deaths of eagles and falcons. I hope this is correct.