Answer:
Below
Explanation:
mRNA is a complimentary copy of a DNA segment (gene) and consequently can be used to deduce the gene sequence. For converting a sequence from mRNA to the original DNA code, apply the rules of complementary base pairing: Cytosine (C) is replaced with Guanine (G) – and vice versa. Uracil (U) is replaced by Adenine (A).
The codon is the three nucleotide sequence in the mRNA that indicates which amino acid should be incorporated in the growing polypeptide chain. The anticodon is the complementary three nucleotide sequence in the appropriate tRNA.
Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
I do not see any experiment, your gonna have to post a picture of it or type out what it is.
The comparison and contrast between the raft and the shore has something to do with freedom. On the river, Huck and Jim are free from the legal, societal and cultural structures which is the opposite if they were on the shore. However, their freedom on the raft was only for a short period of time. When<span> they went back on the shore, they were once again forced to comply with the </span>laws. The<span> similarities between the raft and the shore </span>are<span> natural. They are both are connected with geographical features.</span><span> </span>
D) A & B
it protects the seed against any damage or harmful weather conditions and also helps in dispersal of the seed through different means.
