Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
A as they used they used if while the others are fact
Answer: The carbon cycle is the cycle by which carbon is exchanged among the biosphere, pedosphere, geosphere, hydrosphere, and atmosphere of the Earth. Steps of photosynthesis is absorption of light, electron. transport leading to the reduction, generation of ATP (adenosine triphosphate), and conversion of CO2 into carbohydrates.
Explanation:
Answer:
Stamen/Pistil
Explanation:
Flowering plants also called Angiosperms are a group of plants characterized by the possession of flowers used for sexual reproduction. The flowers contains the male part and female part. The male part of a flower is collectively called STAMEN while the female part is collectively called PISTIL or STYLE.
The STAMEN is responsible for the production of the male reproductive structure called POLLEN while the PISTIL is responsible for the production of female reproductive structure called OVARY. During reproduction, pollen (containing male gametes, sperm) from the STAMEN are transferred to the stigma (receptive part) of the STYLE in a process called POLLINATION.
