Helicases move along one strand of the duplex and push the opposing strand aside. They either migrate in the 3'-5' or 5'-3' orientation along the DNA or RNA (some can move in either direction).
- Living things need helicases, which are enzymes, to separate the double-stranded DNA in this instance from other nucleic acid strands.
- Helicases accomplish this by severing the hydrogen bonds that link the two strands.
- The DNA double helix's two strands are initially separated by an enzyme known as a DNA helicase.
- Each DnaB helicase winds the parental duplex by encircling one strand of the duplex DNA and moving in the 5′ to 3′ direction with respect to this ssDNA.
- Primers are created on occasion when DnaB interacts with primase.
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C because it has the highest rate of reaction at 37 Celsius
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In a normal double strand DNA, adenine (A) on one strand only pair with thymine (T) on the other strand because the two syrands are complementary.