Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
The answer is no Human male can not be carrier for the red green color blindness.
Explanation:
Red green color blindness is a sex linked disorder that is recessive in nature. If an individual male have one copy of X gene with recessive allele of the trait he will be color blind because male have only one copy of X chromosomes and one copy of Y chromosome. So, the recessive X chromosome will express itself in absence of dominant X chromosome.
For being a carrier one should be heterozygous For this gene and its not possible in males as they have only one copy of X chromosome.
Thus, the correct answer is No.
Answer:
D. the homologous chromosomes are paired AND the spindle is formed
Explanation:
Meiosis is the type of cell division that results in four daughter cells with each having a reduced number of chromosomes (by half). Both meiosis and mitosis occur in different stages including prophase, metaphase, anaphase etc but meiosis occurs in two series of division processes called meiosis I and meiosis II.
In prophase I of meiosis, homologous chromosomes, which are similar but non-identical chromosomes from male and female parents pair to form tetrad or bivalent. This pairing of homologous chromosome is unique and peculiar to MEIOSIS but never mitosis.
