Answer: adding heat to the ligation reaction to provide energy to the system, instead of ATP.
Explanation:
Ligation is referred to as an act of joining, and the term refers in biology to an enzymatic reaction that joins a covalent bond between two biomolecules. The application of DNA ligation in research in molecular biology is defined in this video.
Double layered nuclear, the outer nuclear membrane is continuous with the membrane of the rough endoplasmic reticulum (ER), it also continues with the inner nuclear membranes since the two layers are fused together at a numerous tiny holes called nuclear pores that perforate the nuclear enevelope
I say A not sure tho but I did get a grade 5 in biology
Microvilli is the correct term to fill your blank in.