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Airida [17]
3 years ago
11

Your labmate's ligation reaction didn't work. The regular ATP had run out, so he fueled the reaction with dATP from the PCR kit.

What advice can you give him
Biology
1 answer:
V125BC [204]3 years ago
5 0

Answer: adding heat to the ligation reaction to provide energy to the system, instead of ATP.

Explanation:

Ligation is referred to as an act of joining, and the term refers in biology to an enzymatic reaction that joins a covalent bond between two biomolecules. The application of DNA ligation in research in molecular biology is defined in this video.

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