Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
It makes sense that increasing the rate of plastics recycling is helpful for eliminating waste. Think about it, when something can be reused, then it should be. Recycling allows this to happen and prevents landfills from being filled too quickly.
Answer:
The legal term for an advance directive that specifies an individual's end-of-life wishes for medical treatment without necessarily appointing a designee to make legal decisions for him or her is called a living will
Explanation:
A living will is made when alive and it comprises of how one intend to distribute his/her wealth without any interfere by others decision even when dead. The documents must have been signed when alive by the individual
Answer:
red blood cells (RBCs) misfolding and convert in sickle shapevfrom the donut with out hole shape.
Explanation:
It is based on the principle of continuous improvement. The standard enables companies to define what a quality product should be and how it should meet customer needs.
